DEAE Columns
SMT-DEAE [Di-Ethyl-Amino-Ethyl] column provides a unique chemically attached hydrophilic, weak anion exchange type, functional surface desirable for the separation of many biomolecules such as proteins, nucleotides, oligonucleotides, polynucleotides, high molecular weight RNA’s and plasmid DNA’s. The technique of SAM is used in the bonding process to significantly increase the functional ligand density.
SMT-DEAE is silica based and the packing material is mechanically stable at high flow rates and high pressures up to 6,000 psi. SMT-DEAE packing does not swell with organic solvents, salts, or pH gradients.
Special features of SMT-DEAE packing material include:
Fast reequilibration and very negligible non-specific protein interaction.
High density tertiary amine functional groups that provide better selectivity and recovery compared to conventional DEAE.
Highly stable silica-based anion exchange type packing material; Minimal compressibility and will not swell in organic solvents or in the presence of ion pairing reagents.